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Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity
Author(s) -
Tanou Georgia,
Job Claudette,
Rajjou Loïc,
Arc Erwann,
Belghazi Maya,
Diamantidis Grigorios,
Molassiotis Athannasios,
Job Dominique
Publication year - 2009
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.04000.x
Subject(s) - salinity , nitric oxide , acclimatization , protein carbonylation , hydrogen peroxide , sodium nitroprusside , abiotic stress , chemistry , abiotic component , nitrosylation , s nitrosylation , botany , biochemistry , biology , oxidative stress , enzyme , ecology , cysteine , lipid peroxidation , organic chemistry , gene
Summary Hydrogen peroxide (H 2 O 2 ) and nitric oxide (˙NO) are key reactive species in signal transduction pathways leading to activation of plant defense against biotic or abiotic stress. Here, we investigated the effect of pre‐treating citrus plants ( Citrus aurantium L.) with either of these two molecules on plant acclimation to salinity and show that both pre‐treatments strongly reduced the detrimental phenotypical and physiological effects accompanying this stress. A proteomic analysis disclosed 85 leaf proteins that underwent significant quantitative variations in plants directly exposed to salt stress. A large part of these changes was not observed with salt‐stressed plants pre‐treated with either H 2 O 2 or sodium nitroprusside (SNP; a ˙NO‐releasing chemical). We also identified several proteins undergoing changes either in their oxidation (carbonylation; 40 proteins) and/or S ‐nitrosylation (49 proteins) status in response to salinity stress. Both H 2 O 2 and SNP pre‐treatments before salinity stress alleviated salinity‐induced protein carbonylation and shifted the accumulation levels of leaf S ‐nitrosylated proteins to those of unstressed control plants. Altogether, the results indicate an overlap between H 2 O 2 ‐ and ˙NO‐signaling pathways in acclimation to salinity and suggest that the oxidation and S ‐nitrosylation patterns of leaf proteins are specific molecular signatures of citrus plant vigour under stressful conditions.