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The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root
Author(s) -
Huang Lixia,
Yang Songguang,
Zhang Shengchun,
Liu Ming,
Lai Jianbin,
Qi Yanli,
Shi Songfeng,
Wang Jinxiang,
Wang Yaqin,
Xie Qi,
Yang Chengwei
Publication year - 2009
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.03992.x
Subject(s) - arabidopsis , biology , bimolecular fluorescence complementation , microbiology and biotechnology , ubiquitin ligase , cytokinin , meristem , mutant , complementation , dna ligase , cell division , root hair , cell growth , cell cycle , arabidopsis thaliana , gene , genetics , cell , ubiquitin , auxin
Summary hMMS21 is a SUMO E3 ligase required for the prevention of DNA damage‐induced apoptosis, and acts by facilitating DNA repair in human cells. The Arabidopsis genome contains a putative MMS21 homologue capable of interacting with the SUMO E2 conjugating enzyme AtSCE1a, as indicated by a yeast two‐hybrid screen and bimolecular fluorescence complementation experiments. In vitro and in vivo data demonstrated that AtMMS21 was a SUMO E3 ligase. We identified the Arabidopsis AtMMS21 null T‐DNA insertion mutant mms21‐1 , which had a short‐root phenotype, and affected cell proliferation in the apical root meristem, as indicated by impaired expression of the cell division marker CYCB1:GUS in mms21‐1 roots. The mms21‐1 roots had reduced responses to exogenous cytokinins, and decreased expression of the cytokinin‐induced genes ARR3 , ARR4 , ARR5 and ARR7 , compared with the wild type. Thus, our findings suggest that the AtMMS21 gene is involved in root development via cell‐cycle regulation and cytokinin signalling.