Premium
Arabidopsis 14‐3‐3 lambda is a positive regulator of RPW8‐mediated disease resistance
Author(s) -
Yang Xiaohua,
Wang Wenming,
Coleman Mark,
Orgil Undral,
Feng Jiayue,
Ma Xianfeng,
Ferl Robert,
Turner John G.,
Xiao Shunyuan
Publication year - 2009
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.03978.x
Subject(s) - powdery mildew , arabidopsis , salicylic acid , biology , plant disease resistance , mildew , fungal disease , mutant , genetics , arabidopsis thaliana , gene , botany , microbiology and biotechnology
Summary The RPW8 locus from Arabidopsis thaliana Ms‐0 includes two functional paralogous genes ( RPW8.1 and RPW8.2 ) and confers broad‐spectrum resistance via the salicylic acid‐dependent signaling pathway to the biotrophic fungal pathogens Golovinomyces spp. that cause powdery mildew diseases on multiple plant species. To identify proteins involved in regulation of the RPW8 protein function, a yeast two‐hybrid screen was performed using RPW8.2 as bait. The 14‐3‐3 isoform lambda (designated GF14λ) was identified as a potential RPW8.2 interactor. The RPW8.2–GF14λ interaction was specific and engaged the C‐terminal domain of RPW8.2, which was confirmed by pulldown assays. The physiological impact of the interaction was revealed by knocking down GF14λ by T‐DNA insertion, which compromised basal and RPW8‐mediated resistance to powdery mildew. In addition, over‐expression of GF14λ resulted in hypersensitive response‐like cell death and enhanced resistance to powdery mildew via the salicylic acid‐dependent signaling pathway. The results from this study suggest that GF14λ may positively regulate the RPW8.2 resistance function and play a role in enhancing basal resistance in Arabidopsis.