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The GLABRA2 homeodomain protein directly regulates CESA5 and XTH17 gene expression in Arabidopsis roots
Author(s) -
TominagaWada Rumi,
Iwata Mineko,
Sugiyama Junji,
Kotake Toshihisa,
Ishida Tetsuya,
Yokoyama Ryusuke,
Nishitani Kazuhiko,
Okada Kiyotaka,
Wada Takuji
Publication year - 2009
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.03976.x
Subject(s) - arabidopsis , root hair , mutant , cell wall , gene , homeobox , xyloglucan , biology , arabidopsis thaliana , mutation , wild type , genetics , lateral root , secondary cell wall , gene expression , microbiology and biotechnology , botany
Summary Arabidopsis root hair formation is determined by the patterning genes CAPRICE ( CPC ), GLABRA3 ( GL3 ), WEREWOLF ( WER ) and GLABRA2 ( GL2 ), but little is known about the later changes in cell wall material during root hair formation. A combined Fourier‐transform infrared microspectroscopy–principal components analysis (FTIR‐PCA) method was used to detect subtle differences in the cell wall material between wild‐type and root hair mutants in Arabidopsis. Among several root hair mutants, only the gl2 mutation affected root cell wall polysaccharides. Five of the 10 genes encoding cellulose synthase ( CESA1 – 10 ) and 4 of 33 xyloglucan endotransglucosylase ( XTH1 – 33 ) genes in Arabidopsis are expressed in the root, but only CESA5 and XTH17 were affected by the gl2 mutation. The L1‐box sequence located in the promoter region of these genes was recognized by the GL2 protein. These results indicate that GL2 directly regulates cell wall‐related gene expression during root development.