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Modulation of 1 O 2 ‐mediated retrograde signaling by the PLEIOTROPIC RESPONSE LOCUS 1 (PRL1) protein, a central integrator of stress and energy signaling
Author(s) -
Baruah Aiswarya,
Šimková Klára,
Hincha Dirk K.,
Apel Klaus,
Laloi Christophe
Publication year - 2009
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.03935.x
Subject(s) - retrograde signaling , mutant , signal transduction , gene , biology , microbiology and biotechnology , arabidopsis , locus (genetics) , gene expression , genetics
Summary Shortly after the release of singlet oxygen ( 1 O 2 ) in chloroplasts, changes in nuclear gene expression occur in the conditional flu mutant of Arabidopsis that reveal a rapid transfer of signals from the plastid to the nucleus. Extensive genetic screens aimed at identifying constituents involved in 1 O 2 ‐mediated plastid‐to‐nucleus signaling have failed to identify extraplastidic signaling components. This finding suggests that 1 O 2 ‐mediated signals are not translocated to the nucleus via a single linear pathway, but rather through a signaling network that is difficult to block by single mutations. The complexity of this signaling network has been tackled by mutagenizing a transgenic flu line expressing the luciferase reporter gene under the control of the promoter of a 1 O 2 ‐responsive AAA‐ATPase gene (At3g28580) and isolating second site mutants that constitutively express the reporter gene at a high level. One of the mutants was shown by map‐based cloning and sequencing to contain a single amino acid change in the PLEIOTROPIC RESPONSE LOCUS 1 (PRL1) protein. PRL1 suppresses the expression of AAA‐ATPase and other 1 O 2 ‐responsive genes. PRL1 seems to play a major role in modulating responses of plants to environmental changes by interconnecting 1 O 2 ‐mediated retrograde signaling with other signaling pathways.