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Mutation of the rice gene PAIR3 results in lack of bivalent formation in meiosis
Author(s) -
Yuan Wenya,
Li Xingwang,
Chang Yuxiao,
Wen Ruoyu,
Chen Guoxing,
Zhang Qifa,
Wu Changyin
Publication year - 2009
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.03870.x
Subject(s) - synapsis , homologous chromosome , biology , meiosis , genetics , bivalent (engine) , synaptonemal complex , homologous recombination , chromosomal crossover , genetic recombination , gene , recombination , chemistry , organic chemistry , metal
Summary Meiosis is essential for eukaryotic sexual reproduction and important for genetic diversity among individuals. Although a number of genes regulating homologous chromosome pairing and synapsis have been identified in the plant kingdom, their molecular basis remains poorly understood. In this study, we identified a novel gene, PAIR3 ( HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS 3 ), required for homologous chromosome pairing and synapsis in rice. Two independent alleles, designated pair3‐1 and pair3‐2 , were identified in our T‐DNA insertional mutant library which could not form bivalents due to failure of homologous chromosome pairing and synapsis at diakinesis, resulting in sterility in both male and female gametes. Suppression of PAIR3 by RNAi produced similar results to the T‐DNA insertion lines. PAIR3 encodes a protein that contains putative coiled‐coil motifs, but does not have any close homologs in other organisms. PAIR3 is preferentially expressed in reproductive organs, especially in pollen mother cells and the ovule tissues during meiosis. Our results suggest that PAIR3 plays a crucial role in homologous chromosome pairing and synapsis in meiosis.