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Stress‐induced changes in the Arabidopsis thaliana transcriptome analyzed using whole‐genome tiling arrays
Author(s) -
Zeller Georg,
Henz Stefan R.,
Widmer Christian K.,
Sachsenberg Timo,
Rätsch Gunnar,
Weigel Detlef,
Laubinger Sascha
Publication year - 2009
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.03835.x
Subject(s) - transcriptome , arabidopsis thaliana , biology , tiling array , gene , genetics , genome , arabidopsis , abiotic stress , computational biology , pseudogene , gene expression , mutant
Summary The responses of plants to abiotic stresses are accompanied by massive changes in transcriptome composition. To provide a comprehensive view of stress‐induced changes in the Arabidopsis thaliana transcriptome, we have used whole‐genome tiling arrays to analyze the effects of salt, osmotic, cold and heat stress as well as application of the hormone abscisic acid (ABA), an important mediator of stress responses. Among annotated genes in the reference strain Columbia we have found many stress‐responsive genes, including several transcription factor genes as well as pseudogenes and transposons that have been missed in previous analyses with standard expression arrays. In addition, we report hundreds of newly identified, stress‐induced transcribed regions. These often overlap with known, annotated genes. The results are accessible through the Arabidopsis thaliana Tiling Array Express (At‐TAX) homepage, which provides convenient tools for displaying expression values of annotated genes, as well as visualization of unannotated transcribed regions along each chromosome.