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Plant extracellular ATP signalling by plasma membrane NADPH oxidase and Ca 2+ channels
Author(s) -
Demidchik Vadim,
Shang Zhonglin,
Shin Ryoung,
Thompson Elinor,
Rubio Lourdes,
Laohavisit Anuphon,
Mortimer Jennifer C.,
Chivasa Stephen,
Slabas Antoni R.,
Glover Beverley J.,
Schachtman Daniel P.,
Shabala Sergey N.,
Davies Julia M.
Publication year - 2009
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.03830.x
Subject(s) - extracellular , cytosol , nadph oxidase , microbiology and biotechnology , plasma membrane ca2+ atpase , reactive oxygen species , biochemistry , biology , adenosine triphosphate , signal transduction , chemistry , biophysics , atpase , enzyme
Summary Extracellular ATP regulates higher plant growth and adaptation. The signalling events may be unique to higher plants, as they lack animal purinoceptor homologues. Although it is known that plant cytosolic free Ca 2+ can be elevated by extracellular ATP, the mechanism is unknown. Here, we have studied roots of Arabidopsis thaliana to determine the events that lead to the transcriptional stress response evoked by extracellular ATP. Root cell protoplasts were used to demonstrate that signalling to elevate cytosolic free Ca 2+ is determined by ATP perception at the plasma membrane, and not at the cell wall. Imaging revealed that extracellular ATP causes the production of reactive oxygen species in intact roots, with the plasma membrane NADPH oxidase AtRBOHC being the major contributor. This resulted in the stimulation of plasma membrane Ca 2+ ‐permeable channels (determined using patch‐clamp electrophysiology), which contribute to the elevation of cytosolic free Ca 2+ . Disruption of this pathway in the AtrbohC mutant impaired the extracellular ATP‐induced increase in reactive oxygen species (ROS), the activation of Ca 2+ channels, and the transcription of the MAP kinase3 gene that is known to be involved in stress responses. This study shows that higher plants, although bereft of purinoceptor homologues, could have evolved a distinct mechanism to transduce the ATP signal at the plasma membrane.