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TECHNICAL ADVANCE: Free‐flow electrophoresis for purification of plant mitochondria by surface charge
Author(s) -
Eubel Holger,
Lee Chun Pong,
Kuo John,
Meyer Etienne H.,
Taylor Nicolas L.,
Millar A. Harvey
Publication year - 2007
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2007.03253.x
Subject(s) - electrophoresis , proteome , free flow electrophoresis , differential centrifugation , mitochondrion , centrifugation , chromatography , plastid , organelle , biology , chemistry , biochemistry , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , gene , enzyme , chloroplast
Summary Sample purity is the key for a successful in‐depth analysis of any given subcellular proteome. The suitability of free‐flow electrophoresis to assist conventional, centrifugation‐based techniques in the preparation of plant mitochondria from green and non‐green tissue was assessed by various means, including functional assays, immunoblots, electron microscopy and differential gel electrophoresis. Results indicated a significant increase in purity of the mitochondrial samples, highlighted specific contaminants previously reported as mitochondrial proteins, and also pointed to new means for separating plastids and peroxisomes from mitochondria in plant organellar extracts by exploiting differences in surface charge. This approach has the potential to allow a deeper and more comprehensive investigation of the Arabidopsis organellar proteomes, by providing a second dimension of separation based on surface charge in addition to conventional centrifugation purification protocols relying on size and density.