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Characterization of Arabidopsis ABCG11/WBC11, an ATP binding cassette (ABC) transporter that is required for cuticular lipid secretion †
Author(s) -
Bird David,
Beisson Fred,
Brigham Alexandra,
Shin John,
Greer Stephen,
Jetter Reinhard,
Kunst Ljerka,
Wu Xuemin,
Yephremov Alexander,
Samuels Lacey
Publication year - 2007
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2007.03252.x
Subject(s) - arabidopsis , cutin , mutant , atp binding cassette transporter , arabidopsis thaliana , biology , microbiology and biotechnology , secretion , biochemistry , transporter , gene
Summary ABCG11/WBC11, an ATP binding cassette (ABC) transporter from Arabidopsis thaliana , is a key component of the export pathway for cuticular lipids. Arabidopsis wbc11 T‐DNA insertional knock‐out mutants exhibited lipidic inclusions inside epidermal cells similar to the previously characterized wax transporter mutant cer5 , with a similar strong reduction in the alkanes of surface waxes. Moreover, the wbc11 knock‐out mutants also showed defects not present in cer5 , including post‐genital organ fusions, stunted growth and a reduction in cutin load on the plant surface. A mutant line previously isolated in a forward genetics screen, called permeable leaves 1 ( pel1 ), was identified as an allele of ABCG11/WBC11. The double knock‐out wbc11 cer5 exhibited the same morphological and biochemical phenotypes as the wbc11 knock‐out. A YFP‐WBC11 fusion protein rescued a T‐DNA knock‐out mutant and was localized to the plasma membrane. These results show that WBC11 functions in secretion of surface waxes, possibly by interacting with CER5. However, unlike ABCG12/CER5 , ABCG11/WBC11 is important to the normal process of cutin formation.