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Genetic interaction between glabra3‐shapeshifter and siamese in Arabidopsis thaliana converts trichome precursors into cells with meristematic activity
Author(s) -
Marks M. David,
Gilding Edward,
Wenger Jonathan P.
Publication year - 2007
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2007.03243.x
Subject(s) - biology , mutant , ectopic expression , genetics , gene , wild type , allele , basic helix loop helix , trichome , transcription factor , locus (genetics) , microbiology and biotechnology , transcription (linguistics) , myb , dna binding protein , botany , linguistics , philosophy
Summary The identity of many genes required for trichome differentiation is known. This paper describes a novel interaction between mutant alleles of two such genes. One of the alleles, called gl3‐sst , is derived from the GL3 locus, which encodes a basic helix‐loop‐helix type transcription factor. The mutation in the gl3‐sst protein modifies its ability to form a complex with the GL1 protein (a MYB transcription factor required for trichome formation), leading to changes in gene expression compared with wild type during gl3‐sst mutant trichome development. The other mutant allele, sim , is a likely loss of function allele derived from the SIM locus, which is predicted to encode a negative regulator of D‐type cyclin activity. The gl3‐sst sim double mutant exhibits mounds of cells derived from the proliferation of single trichome precursors. The ectopic expression of a D‐type cyclin gene in gl3‐sst mimics the double mutant phenotype. Thus, an interaction between altered trichome gene expression caused by the gl3‐sst mutation and relaxed regulation of D‐type cyclin activity in the double mutant converted a non‐dividing cell into a novel highly proliferating cell type.