Premium
A Pseudomonas syringae pv. tomato DC3000 mutant lacking the type III effector HopQ1‐1 is able to cause disease in the model plant Nicotiana benthamiana
Author(s) -
Wei ChiaFong,
Kvitko Brian H.,
Shimizu Rena,
Crabill Emerson,
Alfano James R.,
Lin NaiChun,
Martin Gregory B.,
Huang HsiouChen,
Collmer Alan
Publication year - 2007
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2007.03126.x
Subject(s) - nicotiana benthamiana , pseudomonas syringae , effector , biology , arabidopsis , type three secretion system , hypersensitive response , mutant , virulence , microbiology and biotechnology , arabidopsis thaliana , pathogen , virology , gene , genetics , plant disease resistance
Summary The model pathogen Pseudomonas syringae pv. tomato DC3000 causes bacterial speck in tomato and Arabidopsis, but Nicotiana benthamiana , an important model plant, is considered to be a non‐host. Strain DC3000 injects approximately 28 effector proteins into plant cells via the type III secretion system (T3SS). These proteins were individually delivered into N. benthamiana leaf cells via T3SS‐proficient Pseudomonas fluorescens , and eight, including HopQ1‐1, showed some capacity to cause cell death in this test. Four gene clusters encoding 13 effectors were deleted from DC3000: cluster II ( hopH1 , hopC1 ), IV ( hopD1 , hopQ1‐1 , hopR1 ), IX ( hopAA1‐2 , hopV1 , hopAO1 , hopG1 ), and native plasmid pDC3000A ( hopAM1‐2 , hopX1 , hopO1‐1 , hopT1‐1 ). DC3000 mutants deleted for cluster IV or just hopQ1‐1 acquired the ability to grow to high levels and produce bacterial speck lesions in N. benthamiana . HopQ1‐1 showed other hallmarks of an avirulence determinant in N. benthamiana : expression in the tobacco wildfire pathogen P. syringae pv. tabaci 11528 rendered this strain avirulent in N. benthamiana , and elicitation of the hypersensitive response in N. benthamiana by HopQ1‐1 was dependent on SGT1. DC3000 polymutants involving other effector gene clusters in a hopQ1‐1 ‐deficient background revealed that clusters II and IX contributed to the severity of lesion symptoms in N. benthamiana , as well as in Arabidopsis and tomato. The results support the hypothesis that the host ranges of P. syringae pathovars are limited by the complex interactions of effector repertoires with plant anti‐effector surveillance systems, and they demonstrate that N. benthamiana can be a useful model host for DC3000.