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Time‐lapse analysis of stem‐cell divisions in the Arabidopsis thaliana root meristem
Author(s) -
Campilho Ana,
Garcia Bernardo,
Toorn Henk v.d.,
Wijk Henk v.,
Campilho Aurélio,
Scheres Ben
Publication year - 2006
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2006.02892.x
Subject(s) - meristem , arabidopsis , arabidopsis thaliana , cell division , biology , stem cell , microbiology and biotechnology , division (mathematics) , asymmetric cell division , botany , root (linguistics) , cell , gene , genetics , shoot , mathematics , arithmetic , mutant , linguistics , philosophy
Summary In the Arabidopsis root, asymmetric stem‐cell divisions produce daughters that form the different root cell types. Here we report the establishment of a confocal tracking system that allows the analysis of numbers and orientations of cell divisions in root stem cells. The system provides direct evidence that stem cells have lower division rates than cells in the proximal meristem. It also allows tracking of cell division timing, which we have used to analyse the synchronization of root cap divisions. Finally, it gives new insights into lateral root cap formation: epidermal stem‐cell daughters can rotate the orientation of the division plane like the stem cell.