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Mutations in the eIF(iso)4G translation initiation factor confer high resistance of rice to Rice yellow mottle virus
Author(s) -
Albar Laurence,
BangratzReyser Martine,
Hébrard Eugénie,
Ndjiondjop MarieNoëlle,
Jones Monty,
Ghesquière Alain
Publication year - 2006
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2006.02792.x
Subject(s) - biology , oryza sativa , genetics , gene , locus (genetics) , virus , genetically modified rice , allele , transgene , genetically modified crops
Summary We report here evidence of the role that the isoform of the eukaryotic translation initiation factor 4G (eIF(iso)4G) plays in naturally occurring resistance in plant/virus interactions. A genetic and physical mapping approach was developed to isolate the Rymv 1 locus controlling the high recessive resistance to Rice yellow mottle virus (RYMV) in the rice ( Oryza sativa ) variety Gigante. The locus was mapped to a 160‐kb interval containing a gene from the eIF(iso)4G family. The stable transformation of a resistant line with the cDNA of this gene, derived from a susceptible variety, resulted in the loss of resistance in transgenic plants. The allelic variability of this gene was analysed in three resistant and 17 susceptible varieties from different cultivated rice species or subspecies. Compared with susceptible varieties, resistant varieties present specific alleles, characterized by either amino acid substitutions or short amino‐acid deletions in the middle domain of the protein. The structure of this domain was modelled and showed that the substitutions were clustered on a small surface patch. This suggests that this domain may be involved in an interaction with the virus.