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Prohibitin is involved in mitochondrial biogenesis in plants
Author(s) -
Ahn Chang Sook,
Lee Jeong Hee,
Reum Hwang A,
Kim Woo Taek,
Pai HyunSook
Publication year - 2006
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2006.02726.x
Subject(s) - prohibitin , biology , microbiology and biotechnology , mitochondrion , senescence , mitochondrial biogenesis , programmed cell death , nicotiana benthamiana , mitochondrial dna , biogenesis , gene silencing , biochemistry , gene , apoptosis
Summary Prohibitin, which consists of two subunits PHB1 and PHB2, plays a role in cell‐cycle progression, senescence, apoptosis, and maintenance of mitochondrial function in mammals and yeast. In this study, we examined the role of prohibitins in plants by using virus‐induced gene silencing (VIGS) of two prohibitin subunit genes of Nicotiana benthamiana , designated NbPHB1 and NbPHB2 . NbPHB1 and NbPHB2 were targeted to the mitochondria, and their gene expression was suppressed during senescence. VIGS of NbPHB2 caused severe growth inhibition, leaf yellowing and symptoms of cell death, whereas VIGS of NbPHB1 resulted in a milder phenotype. At the cellular level, depletion of these subunits affected mitochondria by severely reducing their number and/or mass, and by causing morphological and physiological abnormalities. Suppression of prohibitin function resulted in a 10‐ to 20‐fold higher production of reactive oxygen species and induced premature leaf senescence. Finally, disruption of prohibitin function rendered the plants more susceptible to various oxidative stress‐inducing reagents, including H 2 O 2 , paraquat, antimycin A and salicylic acid. These results suggest that prohibitins play a crucial role in mitochondrial biogenesis and protection against stress and senescence in plant cells.