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Rice plants take up iron as an Fe 3+ ‐phytosiderophore and as Fe 2+
Author(s) -
Ishimaru Yasuhiro,
Suzuki Motofumi,
Tsukamoto Takashi,
Suzuki Kazumasa,
Nakazono Mikio,
Kobayashi Takanori,
Wada Yasuaki,
Watanabe Satoshi,
Matsuhashi Shinpei,
Takahashi Michiko,
Nakanishi Hiromi,
Mori Satoshi,
Nishizawa Naoko K.
Publication year - 2006
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2005.02624.x
Subject(s) - oryza sativa , mutant , saccharomyces cerevisiae , complementary dna , biology , yeast , epidermis (zoology) , transporter , plant cell , botany , biophysics , biochemistry , microbiology and biotechnology , gene , anatomy
Summary Only graminaceous monocots possess the Strategy II iron (Fe)‐uptake system in which Fe is absorbed by roots as an Fe 3+ ‐phytosiderophore. In spite of being a Strategy II plant, however, rice ( Oryza sativa ) contains the previously identified Fe 2+ transporter OsIRT1. In this study, we isolated the OsIRT2 gene from rice, which is highly homologous to OsIRT1 . Real‐time PCR analysis revealed that OsIRT1 and OsIRT2 are expressed predominantly in roots, and these transporters are induced by low‐Fe conditions. When expressed in yeast ( Saccharomyces cerevisiae ) cells, OsIRT2 cDNA reversed the growth defects of a yeast Fe‐uptake mutant. This was similar to the effect of OsIRT1 cDNA. OsIRT1– and OsIRT2–green fluorescent protein fusion proteins localized to the plasma membrane when transiently expressed in onion ( Allium cepa L.) epidermal cells. OsIRT1 promoter– GUS analysis revealed that OsIRT1 is expressed in the epidermis and exodermis of the elongating zone and in the inner layer of the cortex of the mature zone of Fe‐deficient roots. OsIRT1 expression was also detected in the ccompanion cells. Analysis using the positron‐emitting tracer imaging system showed that rice plants are able to take up both an Fe 3+ ‐phytosiderophore and Fe 2+ . This result indicates that, in addition to absorbing an Fe 3+ ‐phytosiderophore, rice possesses a novel Fe‐uptake system that directly absorbs the Fe 2+ , a strategy that is advantageous for growth in submerged conditions.