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A protocol to analyse cellular dynamics during plant development
Author(s) -
Barbier de Reuille Pierre,
BohnCourseau Isabelle,
Godin Christophe,
Traas Jan
Publication year - 2005
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2005.02576.x
Subject(s) - meristem , computer science , preprocessor , segmentation , protocol (science) , dynamics (music) , artificial intelligence , software , biological system , object (grammar) , confocal microscopy , identification (biology) , confocal , computer vision , computational biology , biology , microbiology and biotechnology , shoot , mathematics , botany , physics , pathology , geometry , medicine , alternative medicine , acoustics , programming language
Summary In vivo microscopy generates images that contain complex information on the dynamic behaviour of three‐dimensional (3D) objects. As a result, adapted mathematical and computational tools are required to help in their interpretation. Ideally, a complete software chain to study the dynamics of a complex 3D object should include: (i) the acquisition, (ii) the preprocessing and (iii) segmentation of the images, followed by (iv) a reconstruction in time and space and (v) the final quantitative analysis. Here, we have developed such a protocol to study cell dynamics at the shoot apical meristem in Arabidopsis. The protocol uses serial optical sections made with the confocal microscope. It includes specially designed algorithms to automate the identification of cell lineage and to analyse the quantitative behaviour of the meristem surface.