Premium
Alteration of TGA factor activity in rice results in enhanced tolerance to Xanthomonas oryzae pv. oryzae
Author(s) -
Fitzgerald Heather A.,
Canlas Patrick E.,
Chern MawSheng,
Ronald Pamela C.
Publication year - 2005
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2005.02457.x
Subject(s) - xanthomonas oryzae , xanthomonas oryzae pv. oryzae , biology , genetically modified rice , transgene , npr1 , arabidopsis , plant disease resistance , systemic acquired resistance , genetically modified crops , mutant , gene , transcription factor , gene silencing , microbiology and biotechnology , xanthomonas , oryza sativa , genetics , medicine , heart failure , natriuretic peptide
Summary In dicotyledonous plants broad‐spectrum resistance to pathogens is established after the induction of the systemic acquired resistance (SAR) response. In Arabidopsis the NPR1 protein can regulate SAR by interacting with members of the TGA class of basic, leucine‐zipper transcription factors to alter pathogenesis‐related (PR) gene expression. Overexpression of ( At ) NPR1 in Arabidopsis enhances resistance to multiple pathogens. Similarly, overexpression of ( At ) NPR1 in rice enhances resistance to the bacterial pathogen, Xanthomonas oryzae pv. oryzae ( Xoo ). These results suggest that components of the ( At ) NPR1 ‐mediated SAR defense response may be conserved between monocots and dicots. To determine whether or not rice TGA factors are involved in disease resistance responses, the effect of altering the function of rice TGA2.1 was analyzed in transgenic plants. Transgenic rice overexpressing an rTGA2.1 mutant, that can no longer bind DNA, and transgenic rice that have the endogenous rTGA2.1 silenced by dsRNA‐mediated silencing were generated. Both types of transgenic rice displayed increased tolerance to Xoo , were dwarfed, and had altered accumulation of PR genes. The results presented in this study suggest that wild‐type rTGA2.1 has primarily a negative role in rice basal defense responses to bacterial pathogens.