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Differing requirements for the Arabidopsis Rad51 paralogs in meiosis and DNA repair
Author(s) -
Bleuyard JeanYves,
Gallego Maria E.,
Savigny Florence,
White Charles I.
Publication year - 2005
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02318.x
Subject(s) - rad51 , homologous recombination , biology , genetics , xrcc3 , meiosis , dna repair , arabidopsis , mutant , dna , chromosome segregation , microbiology and biotechnology , gene , chromosome , genotype , single nucleotide polymorphism
Summary In addition to the recombinase Rad51, vertebrates have five paralogs of Rad51, all members of the Rad51‐dependent recombination pathway. These paralogs form two complexes (Rad51C/Xrcc3 and Rad51B/C/D/Xrcc2), which play roles in somatic recombination, DNA repair and chromosome stability. However, little is known of their possible involvement in meiosis, due to the inviability of the corresponding knockout mice. We have recently reported that the Arabidopsis homolog of one of these Rad51 paralogs (AtXrcc3) is involved in DNA repair and meiotic recombination and present here Arabidopsis lines carrying mutations in three other Rad51 paralogs (AtRad51B, AtRad51C and AtXrcc2). Disruption of any one of these paralogs confers hypersensitivity to the DNA cross‐linking agent Mitomycin C, but not to γ ‐irradiation. Moreover, the atrad51c‐1 mutant is the only one of these to show meiotic defects similar to those of the atxrcc3 mutant, and thus only the Rad51C/Xrcc3 complex is required to achieve meiosis. These results support conservation of functions of the Rad51 paralogs between vertebrates and plants and differing requirements for the Rad51 paralogs in meiosis and DNA repair.