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Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP‐tagged proteins
Author(s) -
Damme Daniël Van,
Bouget FrançoisYves,
Poucke Kris Van,
Inzé Dirk,
Geelen Danny
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02222.x
Subject(s) - cytokinesis , phragmoplast , cell plate , septin , biology , microbiology and biotechnology , cleavage furrow , arabidopsis thaliana , exocyst , microtubule , midbody , green fluorescent protein , protein subcellular localization prediction , actin , mitosis , cell division , gene , genetics , cell , protein subunit , mutant
Summary To identify molecular players implicated in cytokinesis and division plane determination, the Arabidopsis thaliana genome was explored for potential cytokinesis genes. More than 100 open reading frames were selected based on similarity to yeast and animal cytokinesis genes, cytoskeleton and polarity genes, and Nicotiana tabacum genes showing cell cycle‐controlled expression. The subcellular localization of these proteins was determined by means of GFP tagging in tobacco Bright Yellow‐2 cells and Arabidopsis plants. Detailed confocal microscopy identified 15 proteins targeted to distinct regions of the phragmoplast and the cell plate. EB1‐ and MAP65‐like proteins were associated with the plus‐end, the minus‐end, or along the entire length of microtubules. The actin‐binding protein myosin, the kinase Aurora, and a novel cell cycle protein designated T22, accumulated preferentially at the midline. EB1 and Aurora, in addition to other regulatory proteins (homologs of Mob1, Sid1, and Sid2), were targeted to the nucleus, suggesting that this organelle operates as a coordinating hub for cytokinesis.