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Phosphoinositide‐specific phospholipase C is involved in cytokinin and gravity responses in the moss Physcomitrella patens
Author(s) -
Repp Alexander,
Mikami Koji,
Mittmann Franz,
Hartmann Elmar
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02205.x
Subject(s) - physcomitrella patens , cytokinin , microbiology and biotechnology , mutant , bryopsida , biology , wild type , protonema , moss , chloroplast , phospholipase c , extracellular , phototropism , botany , gene , biochemistry , signal transduction , auxin , blue light , physics , optics
Summary The phosphoinositide signalling pathway is important in plant responses to extracellular and intracellular signals. To elucidate the physiological functions of phosphoinositide‐specific phopspholipase C, PI‐PLC, targeted knockout mutants of PpPLC1 , a gene encoding a PI‐PLC from the moss Physcomitrella patens , were generated via homologous recombination. Protonemal filaments of the plc1 lines show a dramatic reduction in gametophore formation relative to wild type: this was accompanied by a loss of sensitivity to cytokinin. Moreover, plc1 appeared paler than the wild type, the result of an altered differentiation of chloroplasts and reduced chlorophyll levels compared with wild type filaments. In addition, the protonemal filaments of plc1 have a strongly reduced ability to grow negatively gravitropically in the dark. These effects imply a significant role for PpPLC1 in cytokinin signalling and gravitropism.