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Tackling the plant proteome: practical approaches, hurdles and experimental tools
Author(s) -
Rose Jocelyn K.C.,
Bashir Sajid,
Giovani James J.,
Jahn Molly M.,
Saravanan Ramu Subramanian
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02182.x
Subject(s) - proteome , proteomics , computational biology , identification (biology) , data science , computer science , biology , microbiology and biotechnology , bioinformatics , botany , genetics , gene
Summary The study of complex biological questions through comparative proteomics is becoming increasingly attractive to plant biologists as the rapidly expanding plant genomic and expressed sequence tag databases provide improved opportunities for protein identification. This review focuses on practical issues associated with comparative proteomic analysis, including the challenges of effective protein extraction and separation from plant tissues, the pros and cons of two‐dimensional gel‐based analysis and the problems of identifying proteins from species that are not recognized models for functional genomic studies. Specific points are illustrated using data from an ongoing study of the tomato and pepper fruit proteomes.