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Phosphate transport in Arabidopsis : Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low‐ and high‐phosphate environments
Author(s) -
Shin Heungsop,
Shin HwaSoo,
Dewbre Gary R.,
Harrison Maria J.
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02161.x
Subject(s) - pi , phosphate , mutant , arabidopsis , phosphorus , transporter , arabidopsis thaliana , function (biology) , nutrient , biology , wild type , inorganic phosphate , biochemistry , gene , microbiology and biotechnology , chemistry , ecology , organic chemistry
Summary Of the mineral nutrients essential for plant growth, phosphorus plays the widest diversity of roles and a lack of phosphorus has profound effects on cellular metabolism. At least eight members of the Arabidopsis Pht1 phosphate (Pi) transporter family are expressed in roots and Pht1;1 and Pht1;4 show the highest transcript levels. The spatial and temporal expression patterns of these two genes show extensive overlap. To elucidate the in planta roles of Pht1;1 and Pht1;4, we identified loss‐of‐function mutants and also created a double mutant, lacking both Pht1;1 and Pht1;4. Consistent with their spatial expression patterns, membrane location and designation as high‐affinity Pi transporters, Pht1;1 and Pht1;4 contribute to Pi transport in roots during growth under low‐Pi conditions. In addition, during growth under high‐Pi conditions, the double mutant shows a 75% reduction in Pi uptake capacity relative to wildtype. Thus, Pht1;1 and Pht1;4 play significant roles in Pi acquisition from both low‐ and high‐Pi environments.