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The S haplotype‐specific F‐box protein gene, SFB , is defective in self‐compatible haplotypes of Prunus avium and P. mume
Author(s) -
Ushijima Koichiro,
Yamane Hisayo,
Watari Akiko,
Kakehi Eiko,
Ikeda Kazuo,
Hauck Nathanael R.,
Iezzoni Amy F.,
Tao Ryutaro
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02154.x
Subject(s) - biology , haplotype , genetics , prunus , locus (genetics) , pollen , gene , mutant , coding region , allele , botany
Summary Many Prunus species, including sweet cherry and Japanese apricot, of the Rosaceae, display an S‐RNase‐based gametophytic self‐incompatibility (GSI). The specificity of this outcrossing mechanism is determined by a minimum of two genes that are located in a multigene complex, termed the S locus, which controls the pistil and pollen specificities. SFB , a gene located in the S locus region, encodes an F‐box protein that has appropriate S haplotype‐specific variation to be the pollen determinant in the self‐incompatibility reaction. This study characterizes SFBs of two self‐compatible (SC) haplotypes, S 4′ and S f , of Prunus . S 4′ of sweet cherry is a pollen‐part mutant (PPM) that was produced by X‐ray irradiation, while S f of Japanese apricot is a naturally occurring SC haplotype that is considered to be a PPM. DNA sequence analysis revealed defects in both SFB 4′ and SFB f . A 4 bp deletion upstream from the HVa coding region of SFB 4′ causes a frame‐shift that produces transcripts of a defective SFB lacking the two hypervariable regions, HVa and HVb. Similarly, the presence of a 6.8 kbp insertion in the middle of the SFB f coding region leads to transcripts for a defective SFB lacking the C‐terminal half that contains HVa and HVb. As all reported SFBs of functional S haplotypes encode intact SFB, the fact that the partial loss‐of‐function mutations in SFB are present in SC mutant haplotypes of Prunus provides additional evidence that SFB is the pollen S gene in GSI in Prunus .