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Catalase deficiency drastically affects gene expression induced by high light in Arabidopsis thaliana
Author(s) -
Vandenabeele Steven,
Vanderauwera Sandy,
Vuylsteke Marnik,
Rombauts Stephane,
Langebartels Christian,
Seidlitz Harald K.,
Zabeau Marc,
Van Montagu Marc,
Inzé Dirk,
Van Breusegem Frank
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02105.x
Subject(s) - catalase , arabidopsis thaliana , arabidopsis , peroxisome , transcriptome , hydrogen peroxide , biology , gene expression , microbiology and biotechnology , microarray analysis techniques , biochemistry , transgene , gene , chemistry , oxidative stress , botany , mutant
Summary In plants, hydrogen peroxide (H 2 O 2 ) plays a major signaling role in triggering both a defense response and cell death. Increased cellular H 2 O 2 levels and subsequent redox imbalances are managed at the production and scavenging levels. Because catalases are the major H 2 O 2 scavengers that remove the bulk of cellular H 2 O 2 , altering their levels allows in planta modulation of H 2 O 2 concentrations. Reduced peroxisomal catalase activity increased sensitivity toward both ozone and photorespiratory H 2 O 2 ‐induced cell death in transgenic catalase‐deficient Arabidopsis thaliana . These plants were used as a model system to build a comprehensive inventory of transcriptomic variations, which were triggered by photorespiratory H 2 O 2 induced by high‐light (HL) irradiance. In addition to an H 2 O 2 ‐dependent and ‐independent type of transcriptional response during light stress, microarray analysis on both control and transgenic catalase‐deficient plants, exposed to 0, 3, 8, and 23 h of HL, revealed several specific regulatory patterns of gene expression. Thus, photorespiratory H 2 O 2 has a direct impact on transcriptional programs in plants.