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The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination
Author(s) -
Heitzeberg Fabian,
Chen IPeng,
Hartung Frank,
Orel Nadiya,
Angelis Karel J.,
Puchta Holger
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02097.x
Subject(s) - homologous recombination , dna repair , mutant , dna damage , biology , complementation , g2 m dna damage checkpoint , dna , genetics , rad52 , gene , microbiology and biotechnology , arabidopsis , homology directed repair , rad51 , nucleotide excision repair , cell cycle checkpoint , cell cycle
Summary Rad17 is involved in DNA checkpoint control in yeast and human cells. A homologue of this gene as well as other genes of the pathway (the 9‐1‐1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA‐damaging agents induce At RAD 17 transcriptionally. At RAD17 mutants show increased sensitivity to the DNA‐damaging chemicals bleomycin and mitomycin C (MMC), which can be reversed by complementation, suggesting that the loss of function of Rad17 disturbs DNA repair in plant cells. Our results are further confirmed by the phenotype of a mutant of the 9‐1‐1 complex (Rad9), which is also sensitive to the same chemicals. At RAD9 and At RAD17 seem to be epistatic as the double mutant is not more sensitive to the chemicals than the single mutants. The mutants show a delay in the general repair of double‐strand breaks (DSBs). However, frequencies of intrachromosomal homologous recombination (HR) are enhanced. Nevertheless, the mutants are proficient for a further induction of HR by genotoxic stresses. Our results indicate that a mutant Rad17 pathway is associated with a general deregulation of DNA repair, which seems to be correlated with a deficiency in non‐homologous DSB repair.