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Nuclear localization and interaction of RolB with plant 14‐3‐3 proteins correlates with induction of adventitious roots by the oncogene rolB
Author(s) -
Moriuchi Hiroshi,
Okamoto Chiho,
Nishihama Ryuichi,
Yamashita Ichiro,
Machida Yasunori,
Tanaka Nobukazu
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2004.02041.x
Subject(s) - green fluorescent protein , mutant , agrobacterium , gene , plasmid , biology , nucleus , nuclear protein , root hair , microbiology and biotechnology , chemistry , genetics , transformation (genetics) , transcription factor
Summary The rooting‐locus gene B ( rolB ) on the T‐DNA of the root‐inducing (Ri) plasmid in Agrobacterium rhizogenes is responsible for the induction of transformed adventitious roots, although the root induction mechanism is unknown. We report here that the RolB protein of pRi1724 (1724RolB) is associated with N icotiana t abacum 14‐3‐3 ‐like protein ωII (Nt14‐3‐3 ωII) in tobacco bright yellow (BY)‐2 cells. Nt14‐3‐3 ωII directly interacts with 1724RolB protein. Green fluorescent protein (GFP)‐fused 1724RolB is localized to the nucleus. GFP‐fused mutant 1724RolB proteins having a deletion or amino acid substitution are unable to interact with Nt14‐3‐3 ωII and also show impaired nuclear localization. Moreover, these 1724RolB mutants show decreased capacity for adventitious root induction. These results suggest that adventitious root induction by 1724RolB protein correlates with its interaction with Nt14‐3‐3 ωII and the nuclear localization of 1724RolB protein.