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Overexpression of LSH1 , a member of an uncharacterised gene family, causes enhanced light regulation of seedling development
Author(s) -
Zhao Li,
Nakazawa Miki,
Takase Tomoyuki,
Manabe Katsushi,
Kobayashi Masatomo,
Seki Motoaki,
Shinozaki Kazuo,
Matsui Minami
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2003.01993.x
Subject(s) - phytochrome , arabidopsis , mutant , hypocotyl , seedling , phytochrome a , biology , gene , blue light , endogeny , arabidopsis thaliana , gene family , genetics , mutation , far red , photomorphogenesis , microbiology and biotechnology , gene expression , botany , red light , biochemistry , physics , optics
Summary Light regulates plant growth and development through a network of endogenous factors. By screening Arabidopsis activation‐tagged lines, we isolated a dominant mutant ( l ight‐dependent s hort h ypocotyls 1 ‐ D ( lsh1‐D )) that showed hypersensitive responses to continuous red (cR), far‐red (cFR) and blue (cB) light and cloned the corresponding gene, LSH1 . LSH1 encodes a nuclear protein of a novel gene family that has homologues in Arabidopsis and rice. The effects of the lsh1‐D mutation were tested in a series of photoreceptor mutant backgrounds. The hypersensitivity to cFR and cB light conferred by lsh1‐D was abolished in a phyA null background ( phyA‐201 ), and the hypersensitivity to cR and cFR light conferred by lsh1‐D was much reduced in the phytochrome‐chromophore synthetic mutant, hy1‐1 (long hypocotyl 1). These results indicate that LSH1 is functionally dependent on phytochrome to mediate light regulation of seedling development.