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Cell cycle‐dependent proteolysis and ectopic overexpression of cyclin B1 in tobacco BY2 cells
Author(s) -
Criqui Marie Claire,
Parmentier Yves,
Derevier Aude,
Shen WenHui,
Dong Aiwu,
Genschik Pascal
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2000.t01-1-.x
Subject(s) - proteolysis , ectopic expression , cell cycle , microbiology and biotechnology , cyclin , plant cell , biology , cell , cyclin b1 , cancer research , genetics , gene , cyclin dependent kinase 1 , biochemistry , enzyme
Summary Activation of cyclin B/Cdc2 kinase complex triggers entry into mitosis in all eukaryotic cells. Although cyclin gene expression has been extensively studied in plants, not much is known at the level of the protein stability and function. Here, we demonstrated by using the highly synchronizable tobacco BY2 cell culture, that endogenous cyclin B1 protein undergoes cell cycle‐dependent proteolysis and is stabilized when the spindle checkpoint has been activated. Furthermore, we established transgenic tobacco BY2 cell cultures expressing under the control of an inducible promoter, cyclin B1 protein as well as its non‐degradable form as fusion proteins with GFP and found that the ectopic expression of these proteins did not dramatically disturb the cell cycle progression. These results indicate that, to a certain extent, cell cycle exit is possible without cyclin B1 proteolysis.