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Application of high‐performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids
Author(s) -
Fraser Paul D.,
Pinto M. Elisabete S.,
Holloway Daniel E.,
Bramley Peter M.
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2000.00896.x
Subject(s) - chromatography , high performance liquid chromatography , chemistry , photodiode , xanthophyll , elution , carotenoid , materials science , biochemistry , optoelectronics
Summary The application of high‐performance liquid chromatography (HPLC) using a C 30 reverse‐phase stationary matrix has enabled the simultaneous separation of carotenes, xanthophylls, ubiquinones, tocopherols and plastoquinones in a single chromatogram. Continuous photodiode array (PDA) detection ensured identification and quantification of compounds upon elution. Applications of the method to the characterization of transgenic and mutant tomato varieties with altered isoprenoid content, biochemical screening of Arabidopsis thaliana , and elucidation of the modes of action of bleaching herbicides are described to illustrate the versatility of the procedure.