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Bigfoot : a new family of MITE elements characterized from the Medicago genus
Author(s) -
Charrier Bénédicte,
Foucher Fabrice,
Kondorosi Eva,
D’AubentonCarafa Yves,
Thermes Claude,
Kondorosi Adam,
Ratet Pascal
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1999.00469.x
Subject(s) - medicago truncatula , biology , transposable element , genome , botany , genetics , lotus japonicus , mite , gene , symbiosis , bacteria , mutant
Summary We have characterized from the legume plant Medicagoa new family of miniature inverted‐repeat transposable elements (MITE), called the Bigfoot transposable elements. Two of these insertion elements are present only in a single allele of two different M. sativa genes. Using a PCR strategy we have isolated 19 other Bigfoot elements from the M. sativa and M. truncatula genomes. They differ from the previously characterized MITEs by their sequence, a target site of 9 bp and a partially clustered genomic distribution. In addition, we show that they exhibit a significantly stable secondary structure. These elements may represent up to 0.1% of the genome of the outcrossing Medicago sativa but are present at a reduced copy number in the genome of the autogamous M. truncatula plant, revealing major differences in the genome organization of these two plants.