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Developmentally regulated expression of the wound‐ and pathogen‐responsive tomato anionic peroxidase in green fruits
Author(s) -
Sherf Bruce A.,
Kolattukudy P.E.
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1993.00829.x
Subject(s) - peroxidase , pathogen , chemistry , botany , microbiology and biotechnology , biology , biochemistry , enzyme
Summary A highly anionic peroxidase (TAP) isolated from tomato was thought to be induced only upon wounding or fungal attack as this protein and its mRNA could not be detected in the vegetative tissues of healthy tomato but accumulated to very high levels in tissues responding to wounding or fungal attack. We now demonstrate a pattern of developmentally regulated expression of the tap gene in the exocarp of green, but not red, tomato fruits. Detaching green fruits from the vine, however, leads to the complete loss of the tap transcript within 72 h. Administration of various growth regulators through the pedicel of detached green fruits revealed that cytokinin was able to sustain a level of tap transcripts in 72 h detached fruits comparable with that found in fruits attached to the vine. Wound‐induced expression of tap was confined to the healing periderm of green fruits but was not detectable in the wound‐tissue of post‐climacteric ripening fruits. These results suggest that the developmental and wound regulated expression of tap may occur by separate or overlapping signal transduction pathways. Treatment of tomato plants and green fruits with 2‐chloro‐ethylphosphonic acid (ethephon) demonstrated that ethylene does not effect the developmental suppression of tap in post‐climacteric fruits, nor does it alter the normal levels of tap transcripts in either healthy or wound‐healing tomato tissues.