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Differential expression of the rol A plant oncogene and its effect on tobacco development
Author(s) -
Carneiro Mauro,
Vilaine Françoise
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1993.00785.x
Subject(s) - biology , gene , mutant , nicotiana tabacum , enhancer , transgene , gene expression , regulation of gene expression , genetics , arabidopsis , promoter , regulatory sequence , microbiology and biotechnology
Summary The rol A gene not only provokes the abnormal traits of wrinkled leaves, shortened internodes, and deficient root growth in tobacco, but also induction of roots in leaf disks in hormone‐free medium. The rol A mRNA is most abundant in the stem of transgenic tobacco, accumulating at fivefold and 50‐fold lower levels in the leaf and the root, respectively. Promoter deletion mutants were constructed and the rol A‐induced abnormal traits were exploited to identify promoter regulatory regions. The removal of specific promoter domains provokes the appearance of new patterns of rol A transcripts in leaves, stems and roots. These new patterns are correlated with the disappearance of specific abnormal trait, thereby identifying three functional domains. Domain A includes a mosaic motif composed of a putative GT‐2 binding site and DUE‐NDE elements. This domain positively regulates rol A gene expression in the leaf and negatively regulates it in the stem and root. Domains B and C, which contain sequences homologous to the OCS enhancer and to ASF‐1 binding sites, respectively, generally activate the rol A gene expression in all tissues. The possible function of these elements on the regulation of the rol A gene is discussed.