Premium
Characterization of a pollen‐specific cDNA from sunflower encoding a zinc finger protein
Author(s) -
Baltz Rachel,
Domon Claire,
Pillay Datta T.N.,
Steinmetz André
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1992.tb00140.x
Subject(s) - complementary dna , biology , gene , zinc finger , genetics , cdna library , rapid amplification of cdna ends , nucleic acid sequence , microbiology and biotechnology , intron , homology (biology) , transcription factor , molecular cloning
We have isolated, via differential hybridization screening of a floral cDNA library from sunflower, a cDNA clone that hybridizes to a 1100 nucleotide‐long mRNA found exclusively in mature pollen grains. The cDNA encodes a 219 amino acid‐long polypeptide containing two potential zinc fingers alternating with two basic domains. A similar organization is found in the erythroid‐specific transcription factor Eryf1 from chicken and its murine homolog GF‐1. The C‐terminus of the protein contains a sixfold repeat of the pentapeptide sequence (S,T,A)(E,D)TQN. These features suggest that the SF3 protein is a transcription factor required for the expression of late pollen genes. The SF3 gene is a member of a multicopy gene family. A genomic copy of the gene has been isolated and sequenced; it is split by four short, AT‐rich introns.