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Random sequencing of cDNA libraries reveals a variety of expressed genes in cultured cells of rice ( Oryza sativa L.)
Author(s) -
Uchimiya Hirofumi,
Kidou Shinichiro,
Shimazaki Tetsuo,
Aotsuka Satoshi,
Takamatsu Susumu,
Nishi Ritsuko,
Hashimoto Hiroshi,
Matsubayashi Yuko,
Kidou Naomi,
Umeda Masaaki,
Kato Atsushi
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1992.01005.x
Subject(s) - genbank , oryza sativa , complementary dna , cdna library , biology , gene , expressed sequence tag , genetics , microbiology and biotechnology
Summary Large scale sequencing of randomly selected cDNA clones was carried out to investigate the feasibility of this method for isolating plant genes. cDNA libraries were made using mRNA prepared from suspension‐cultured cells of rice ( Oryza sativa L.). Partial nucleotide sequences of 830 individual cDNA clones have been determined and compared with the GenBank database. Approximately 8% of the cDNA clones could be identified as particular genes. This method provides the opportunity to isolate large numbers of plant genes.