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Maternal mRNA and early development in Fucus serratus
Author(s) -
Masters A. K.,
Shirras A. D.,
Hetherington A. M.
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1992.00619.x
Subject(s) - messenger rna , rna , polyadenylation , translation (biology) , immunoprecipitation , biology , in vitro , protein biosynthesis , microbiology and biotechnology , chemistry , biochemistry , gene
Summary Intact total and polyadenylated RNA have been isolated from unfertilized eggs of the brown marine macroalga Fucus serratus . The presence of functional messenger RNA has been demonstrated by translation in vitro . The major in‐vitro translation product has an apparent molecular mass of 42 kDa. Immunoprecipitation of translation products of egg RNA using an anti‐actin antibody yields a polypeptide which co‐migrates with this major translation product.