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A Brassica S ‐locus related gene promoter directs expression in both pollen and pistil of tobacco
Author(s) -
Hackett Rachel M.,
Lawrence Michael J.,
Franklin F. Christopher H.
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1992.00613.x
Subject(s) - nicotiana tabacum , biology , gene , brassica oleracea , genetics , locus (genetics) , promoter , gene expression , agrobacterium , genetically modified crops , microbiology and biotechnology , transformation (genetics) , transgene , botany
Summary A portion (1.5 kb) of the promoter region of an S63 S ‐locus related ( SLR ) glycoprotein gene from the sporophytically self‐incompatible species Brassica oleracea was inserted upstream of the β‐glucuronidase (GUS) gene in binary vector pBI101.1. The resulting construct was then introduced into Nicotiana tabacum through Agrobacterium ‐mediated transformation. The expression pattern of GUS under the control of the S63 SLR promoter fragment was found to be similar to that already reported for expression of the GUS gene directed by an S ‐locus specific gene promoter in transgenic N. tabacum . Furthermore, this pattern of expression resembled more closely that reported for S ‐genes of the self‐incompatible species Nicotiana alata , which has a gametophytic self‐incompatibility system, than the predicted pattern of expression of S ‐genes in B. oleracea .