Premium
Artifacts in the localization of GUS activity in anothers of petunia transformed with a CaMV 35S‐GUS construct
Author(s) -
Mascarenhas Joseph P.,
Hamilton Douglas A.
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1992.00405.x
Subject(s) - cauliflower mosaic virus , petunia , staining , pollen , biology , beta glucuronidase , stain , botany , microbiology and biotechnology , vital stain , transgene , genetically modified crops , gene , genetics , gene expression
Summary Blue staining of pollen in transgenic petunia plants containing a β‐glucuronidase gene under the control of a cauliflower mosaic virus 35S promoter, is an artifact caused by the diffusion of reaction products from sites in the another and their dimerization into indigo in pollen. The CaMV 35S promoter is active in various tissues of the another. When pollens from Tradescantia and snapdragon are mixed with transgenic petunia anothers during staining they stain blue, whereas they are colorless when stained alone. In assays for promoter activity in pollen it is essential that pollen is isolated from the another or another section prior to staining for GUS activity, to prevent diffusion artifacts and false staining of pollen.