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A 126 bp fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis
Author(s) -
Atanassova Rossitza,
Chaubet Nicole,
Gigot Claude
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1992.00291.x
Subject(s) - arabidopsis , meristem , transgene , gene , biology , promoter , histone , genetics , gene expression , microbiology and biotechnology , mutant
Summary The tissue‐specific pattern of expression directed by the H4A748 Arabidopsis histone promoter was investigated by analysis of β‐glucuronidase (GUS) activity in transgenic Arabidopsis containing H4A748 ‐GUS gene fusions. As determined by fluorimetric and histochemical tests, the H4A748 promoter directs preferential expression in meristems of young seedlings and adult plants. The low activity found in non‐proliferating tissues may relate to basal constitutive expression of the histone promoter and/or to endo‐reduplication occurring in some tissues. The endogenous histone mRNA levels parallel the GUS activity found in different tissues. Analysis of the regulatory properties of 5′ deleted promoters showed that multiple positive elements exist between −900 and −219 and that the proximal region of the promoter to −219 is sufficient to establish the full tissue‐specific pattern of expression. Further deletion to −93 nearly abolished the promoter activity thus suggesting that the 126 bp fragment located between −219 and −93 contains the elements responsible for the specific expression pattern. The presence of several remarkable sequences within this fragment is discussed.