Premium
Constitutive nitrate reductase: a dominant conditional marker for plant genetics
Author(s) -
Nussaume Laurent,
Vincentz Michel,
Caboche Michel
Publication year - 1991
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1991.00267.x
Subject(s) - chlorate , nitrate reductase , complementary dna , transgene , biology , gene , genetically modified crops , genetics , endogeny , nitrate , botany , microbiology and biotechnology , biochemistry , chemistry , enzyme , inorganic chemistry , ecology
Summary We describe the development of a counter‐selection system based on the use of an engineered nitrate reductase (NR) gene. The engineered gene consists of an NR cDNA placed under the control of the CaMV 35S promoter ( 35S‐NR ). Seedlings and cells derived from transgenic Nicotiana plumbaginifolia plants transformed with 35S‐NR are efficiently killed by the selective agent chlorate on medium containing ammonium as the sole nitrogen source. Under these nitrate‐free conditions wild‐type plants are not affected by chlorate because the endogenous wild‐type Nia gene is not expressed.