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Transcriptional repression of light‐induced flavonoid synthesis by elicitor treatment of cultured parsley cells
Author(s) -
Lozoya Edmundo,
Block Annette,
Lois Rodrigo,
Hahlbrock Klaus,
Scheel Dierk
Publication year - 1991
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1991.00227.x
Subject(s) - chalcone synthase , elicitor , phenylpropanoid , biology , transcription (linguistics) , chalcone , biochemistry , phenylalanine ammonia lyase , reporter gene , biosynthesis , gene expression , gene , microbiology and biotechnology , enzyme , chemistry , peroxidase , linguistics , philosophy , stereochemistry
Summary Cultured parsley (Petroselinum crispum) cells respond differentially to UV‐containing white light and fungal elicitor. Both stimuli activate the transcription of genes encoding enzymes of partly overlapping phenylpropanoid biosynthetic pathways. Irradiation induces vacuolar accumulation of flavonoids, whereas elicitor treatment stimulates the secretion of furanocoumarins. Simultaneous treatment of parsley cells with UV light and elicitor results in quantitative changes in both responses. Irradiation reduces elicitor‐induced furanocoumarin synthesis, possibly by post‐transcriptional mechanisms, whereas elicitor treatment completely blocks the light‐induced accumulation of flavonoids by repressing the transcription of the chalcone synthase gene. We have identified elicitor‐sensitive regions in the chalcone synthase promoter by transient expression analysis of selected promoter constructs linked to the β‐D‐glucuronidase reporter gene in parsley protoplasts. These regions are identical to those that were found to be sufficient for light inducibility of the chalcone synthase promoter.