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Mitochondrial genome organization and expression associated with cytoplasmic male sterility in sunflower ( Helianthus annuus)
Author(s) -
Laver H.K.,
Reynolds S.J.,
Moneger F.,
Leaver C.J.
Publication year - 1991
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.1991.00185.x
Subject(s) - biology , cytoplasmic male sterility , helianthus annuus , genetics , sunflower , mitochondrial dna , gene , sterility , nuclear gene , genome , open reading frame , peptide sequence , agronomy
Summary A comparative investigation of the organization and expression of the mitochondrial genome in fertile and cytoplasmic male sterile (CMS) sunflower (Helianthus annuus) has been undertaken. A region of mitochondrial genome variation between the two phenotypes has been located in the 3′ flanking region of the gene encoding the α subunit of the F 1 ATPase ( atpA ). Physical mapping and sequence analysis have been used to show that a rearrangement involving an inversion and an insertion has occurred immediately downstream of the atpA coding region in the mitochondrial DMA from sterile sunflower. This rearrangement has resulted in the creation of a new open reading frame (ORFc) which is co‐transcribed with atpA in sterile sunflower. In organello labelling of mitochondrial translation products from the two types of sunflower shows that a 15 kDa protein is synthesized by the mitochondria from sterile sunflower but not by those from fertile plants. The ORFc sequence could encode this 15 kDa protein which may be causally related to the CMS phenotype.