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Phylogenetic utility of dynamin and triose phosphate isomerase
Author(s) -
HARDY NATE B.
Publication year - 2007
Publication title -
systematic entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 66
eISSN - 1365-3113
pISSN - 0307-6970
DOI - 10.1111/j.1365-3113.2007.00377.x
Subject(s) - biology , triosephosphate isomerase , dynamin , phylogenetic tree , phylogenetics , evolutionary biology , genetics , computational biology , gene , endocytosis , cell
Dynamin and triose phosphate isomerase (Tpi), two markers suggested as potentially useful for insect phylogenetics, have been sequenced for 12 scale insect taxa (Hemiptera: Coccoidea). Protocols are given for their amplification using conventional polymerase chain reaction, and their phylogenetic utility has been evaluated using several qualitative criteria and a modified version of the partition addition bootstrap alteration approach. Dynamin and Tpi fragments are easy to amplify and are evolving at a rate comparable with widely used nuclear ribosomal markers. The dynamin fragment has a single short intron. The Tpi fragment has three introns. One possible drawback to the use of the dynamin fragment as a phylogenetic marker is that its short length limits accurate modelling of complex substitution processes.