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Gentamicin in Combination with Ascorbic Acid Regulates the severity of S taphylococcus aureus Infection–Induced Septic Arthritis in Mice
Author(s) -
Mal P.,
Dutta S.,
Bandyopadhyay D.,
Dutta K.,
Basu A.,
Bishayi B.
Publication year - 2012
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2012.02766.x
Subject(s) - ascorbic acid , gentamicin , staphylococcus aureus , arthritis , superoxide dismutase , antioxidant , septic arthritis , microbiology and biotechnology , catalase , medicine , necrosis , pharmacology , antibiotics , chemistry , immunology , bacteria , biology , biochemistry , food science , genetics
To study the effects of gentamicin in combination with ascorbic acid on septic arthritis, mice were infected with Staphylococcus aureus ( S. aureus ) and treated with gentamicin, which was given at 5 mg/kg after 24 h of infection, followed by ascorbic acid, given at 20 mg/kg body weight after 2 h of gentamicin treatment. Mice were sacrificed at 3, 9, 15 days post‐infection (dpi). Combined treatment of infected mice with gentamicin and ascorbic acid eradicated the bacteria from the blood, spleen and synovial tissue and showed a significant gross reduction in arthritis, reduced serum levels of tumour necrosis factor alpha ( TNF ‐α) and interferon gamma ( IFN ‐γ). S. aureus ‐infected mice have demonstrated the disturbed antioxidant status measured in terms of cellular antioxidants like reduced glutathione and antioxidant enzymes such as superoxide dismutase ( SOD ) and catalase. The same were ameliorated when the animals were co‐treated with gentamicin along with ascorbic acid.