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Distinct In Vitro Myelopoiesis is Dependent on the Self‐Renewal of Hematopoietic Progenitors
Author(s) -
Hinton R. A.,
Papathanasiou P.,
O’Neill H. C.
Publication year - 2012
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2011.02643.x
Subject(s) - progenitor cell , haematopoiesis , myelopoiesis , spleen , biology , bone marrow , stromal cell , microbiology and biotechnology , immunology , stem cell , embryonic stem cell , progenitor , cancer research , genetics , gene
Spleen and bone marrow (BM) have been shown to contain the progenitors of a novel dendritic‐like antigen‐presenting cell type (L‐DC). These progenitors are also maintained in both long‐term spleen cultures and co‐cultures of spleen or BM over the stromal cell line STX3. We examined mouse foetal liver (FL), rich in hematopoietic stem/progenitor cells (HSC/HPC) after embryonic day (E) 12.5, for the presence of L‐DC progenitors by testing their capacity to colonize STX3 and produce L‐DC. E14.5 FL from wild‐type C57BL/6J mice was found to colonize STX3 and produce L‐DC for 28 days. By contrast, E14.5 FL from Ikaros Plastic mice gave only short‐term production of low numbers of L‐DC between 7 and 14 days of co‐culture. The transient and weak production of L‐DC by FL from Plastic E14.5 mice maps to the loss of self‐renewal capacity amongst HSC. L‐DC progenitors are, therefore, closely aligned with a subset of self‐renewing HSC/HPC in FL.