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Analysis of Properties and Proinflammatory Functions of Cockroach Allergens Per a 1.01s
Author(s) -
He S.,
Zhang Z.,
Zhang H.,
Wei J.,
Yang L.,
Yang H.,
Sun W.,
Zeng X.,
Yang P.
Publication year - 2011
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2011.02571.x
Subject(s) - cockroach , proinflammatory cytokine , immunology , american cockroach , recombinant dna , allergy , allergen , flow cytometry , chemistry , medicine , biology , inflammation , biochemistry , periplaneta , ecology , gene
Cockroaches have been identified as one of the major indoor allergens inducing perennial rhinitis and asthma. Per a 1s are a group of the major allergens from American cockroach. Although Per a 1s are major allergens from American cockroach, factors contributing to the allergenicity of Per a 1s are still poorly defined. To investigate the effects of Per a 1s on the expression of PARs and the release of proinflammatory cytokines from mast cells. Per a 1.0101 and Per a 1.0104 were cloned from American cockroach and then expressed in Eschericia coli . The purified allergens were used to stimulate P815 mast cells, and the expression of protease‐activated receptors (PARs) was determined by real‐time RT‐PCR and flow cytometry. The levels of IL‐4 and IL‐13 in culture media were detected with ELISA. Sera from 80 and 77.3% of cockroach allergy patients reacted to recombinant Per a (rPer a) 1.0101 and rPer a 1.0104, confirming they are major allergens. Both rPer a 1.0101 and rPer a 1.0104 had no enzymatic activity, but rPer a 1.0101 upregulated the expression of PAR‐1 and PAR‐2, and rPer a 1.0104 enhanced the expression of PAR‐1 and PAR‐4 proteins. Both recombinant allergens were able to increase the release of IL‐4 and IL‐13 from P815 mast cells. This is the first study aiming to investigate functions of group 1 allergens of American cockroach. rPer a 1.0101 and rPer a 1.0104 have the capacity to upregulate the expression of PARs and to enhance Th2 cytokine production in mast cells.