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Immunoglobulin Subclass Profiles of Anti‐idiotypic Antibodies to GAD65Ab Differ Between Type 1 Diabetes Patients and Healthy Individuals
Author(s) -
Oak S.,
Radtke J.,
Törn C.,
LandinOlsson M.,
Hampe C. S.
Publication year - 2011
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2011.02565.x
Subject(s) - subclass , autoantibody , antibody , immunology , type 1 diabetes , immunoglobulin g , autoimmunity , medicine , biology , diabetes mellitus , endocrinology
Previously we reported the presence of anti‐idiotypic antibodies (anti‐Id)‐specific to autoantibodies against GAD65 (GAD65Ab) in healthy individuals while the activity of anti‐Id directed to GAD65Ab in type 1 diabetes (T1D) patients was significantly lower. These anti‐Id recognize the antigen‐binding site of GAD65Ab, thus preventing their binding to GAD65. Here, we characterized the IgG subclass profile of these anti‐Id (GAD65Ab specific) and of the associated GAD65Ab themselves. The IgG subclass response of anti‐Id in healthy individuals ( n = 16) was IgG3‐dominated, while in T1D patients ( n = 8) IgG1 was the major IgG subclass. The GAD65Ab bound by anti‐Id in both healthy individuals ( n = 38) and GAD65Ab‐negative T1D patients ( n = 35) showed a predominant rank order of IgG1 > IgG2 > IgG4 > IgG3. However, the frequency of GAD65Ab of the IgG4 subclass was significantly higher in T1D patients ( P < 0.05). We conclude that the IgG subclass profile of anti‐Id (GAD65Ab specific) in healthy individuals differs from that in T1D patients. These differences may provide insights into the development of these antibodies.