Premium
Mitogenic Response and Cytokine Production Induced by Cramoll 1,4 Lectin in Splenocytes of Inoculated Mice
Author(s) -
de Melo C. M. L.,
Melo H.,
Correia M. T. S.,
Coelho L. C. B. B.,
da Silva M. B.,
Pereira V. R. A.
Publication year - 2011
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2010.02490.x
Subject(s) - lectin , splenocyte , mannan binding lectin , concanavalin a , in vitro , nitric oxide , in vivo , cd69 , biology , cytokine , mannose , microbiology and biotechnology , chemistry , immunology , biochemistry , il 2 receptor , endocrinology , cytotoxic t cell
Cramoll 1,4 is a lectin with specific glucose/mannose binding, which is extracted from seeds of Cratylia mollis Mart. Many assays have shown the cytokine expression activity and anti‐inflammatory profile of this lectin. The aim of this study was to evaluate the immunostimulatory response, in vitro , of splenocytes in mice previously inoculated, in vivo , with C. mollis (Cramoll 1,4) and Canavalia ensiformis (Con A) lectins. Results demonstrated higher proliferation indexes induced by Cramoll 1,4 than Con A lectin in relation to all experimental groups. Cramoll 1,4 and Con A also induced high levels of IL‐2, IL‐6, IFN‐γ and nitric oxide production. Moreover, Cramoll 1,4 did not induce apoptosis and stimulated a significant number of cells in the S phase of the cell cycle. Results showed that Cramoll 1,4 lectin induces proliferative response and suggested that this lectin can be used as a mitogenic agent in immunostimulatory assays.