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Interferon‐gamma +874 T/A and Interleukin‐10 ‐1082 A/G Single nucleotide Polymorphism in Egyptian Children with Tuberculosis
Author(s) -
Mosaad Y. M.,
Soliman O. E.,
Tawhid Z. E.,
Sherif D. M.
Publication year - 2010
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2010.02426.x
Subject(s) - genotype , medicine , tuberculosis , mycobacterium tuberculosis , gastroenterology , immunology , single nucleotide polymorphism , interferon gamma , allele , allele frequency , cytokine , pathology , biology , gene , genetics
The aim was to investigate the association of interferon‐gamma (IFN‐γ) +874 T/A and interleukin‐10 (IL‐10)‐1082 A/G single nucleotide polymorphisms with tuberculous infection and post‐BCG lymphadenitis in Egyptian children. IFN‐γ +874 T/A and IL‐10 ‐1082 A/G polymorphism detection by amplification refractory mutation system technique was carried out for 110 patients with TB, 40 patients with post‐BCG lymphadenitis and 118 healthy controls. IFN‐γ +874 A allele was higher in TB and post‐BCG patients than those in healthy controls ( P c = 0.006 and 0.002, respectively). IFN‐γ +874 genotype AA was significantly higher in patients with TB than that in control ( P c = 0.015), in extrapulmonary than patients with pulmonary TB (PTB) ( P c = 0.009), and young children with TB below 5 years ( P c = 0.024). No statistically significant differences were observed between patients with TB and controls for the frequency of IL‐10(‐1082) alleles or genotypes ( P > 0.05); however, a statistically significant difference in the frequency of IL‐10 (‐1082) GG genotype was found between patients with pulmonary and extrapulmonary TB ( P c = 0.003). Low producer IFN‐γ +874 A/A genotype is associated with post‐BCG lymphadenitis and TB disease especially in younger children below 5 years. IL‐10‐1082 G/G genotype did not exhibit significant association except for increased GG frequency in PTB. Both cytokine polymorphisms have no relation to tuberculin reaction in patients with TB.