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Intradermal Delivery of Recombinant Vaccinia Virus Vector DIs Induces Gut‐Mucosal Immunity
Author(s) -
Yoshino N.,
Kanekiyo M.,
Hagiwara Y.,
Okamura T.,
Someya K.,
Matsuo K.,
Ami Y.,
Sato S.,
Yamamoto N.,
Honda M.
Publication year - 2010
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2010.02416.x
Subject(s) - vaccinia , virology , antigen , immunity , immunology , biology , antibody , immune system , intradermal injection , virus , spleen , cellular immunity , humoral immunity , recombinant dna , biochemistry , gene
Antigen‐specific mucosal immunity is generally induced by the stimulation of inductive mucosal sites. In this study, we found that the replication‐deficient vaccinia virus vector, DIs, generates antigen‐specific mucosal immunity and systemic responses. Following intradermal injection of recombinant DIs expressing simian immunodeficiency virus gag (rDIsSIV gag ), we observed increased levels of SIV p27‐specific IgA and IgG antibodies in faecal extracts and plasma samples, and antibody‐forming cells in the intestinal mucosa and spleen of C57BL/6 mice. Antibodies against p27 were not detected in nasal washes, saliva, and vaginal washes. The enhanced mucosal and systemic immunity persisted for 1 year of observation. Induction of Gag‐specific IFN‐γ spot‐forming CD8 + T cells in the spleen, small intestinal intraepithelial lymphocytes, and submandibular lymph nodes was observed in the intradermally injected mice. Heat‐inactivated rDIsSIV gag rarely induced antigen‐specific humoral and T‐helper immunity. Moreover, rDIsSIV gag was detected in MHC class II IA antigen‐positive (IA + ) cells at the injection site. Consequently, intradermal delivery of rDIs effectively induces antigen‐specific humoral and cellular immunity in gut‐mucosal tissues of mice. Our data suggest that intradermal injection of an rDIs vaccine may be useful against mucosally transmitted pathogens.